# Bulk RNA-Seq Analysis Training

{% embed url="<https://youtu.be/8Usk5XkVvXg>" %}

**Agenda with start times:**

* Transfer file to the server (00:02:28)
* Import fastq files (00:03:55)
* Add sample attributes (00:06:00)
* Pre-alignment QA/QC (00:10:45)
* Alignment QC (00:15:30)
* Quantification (00:21:45)
* Filter and normalization (00:32:13)
* Differential analysis and visualization (00:38:50)
* Filter genes to generate a heatmap (00:53:50)
* Biological interpretation (01:08:00)