Bulk RNA-Seq Analysis Training

Agenda with start times:

  • Transfer file to the server (00:02:28)

  • Import fastq files (00:03:55)

  • Add sample attributes (00:06:00)

  • Pre-alignment QA/QC (00:10:45)

  • Alignment QC (00:15:30)

  • Quantification (00:21:45)

  • Filter and normalization (00:32:13)

  • Differential analysis and visualization (00:38:50)

  • Filter genes to generate a heatmap (00:53:50)

  • Biological interpretation (01:08:00)

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