# Creating a list of enriched regions

In this section, we will create a list of peaks significantly enriched in the ChIP sample versus the control sample.

* Select **Create a list of enriched regions** from the *Peak Analysis* section of the *ChIP-Seq* workflow
* Select **Specify New Criteria** (Figure 1)

![](/files/FotuTVlJ4ODLh2aLBrBa)

Figure 1. List creator for ChIP-Seq data allows you to create lists using preset or custom criteria

Configure the new criteria as shown (Figure 2).

* Name the criteria **p-value filtered**
* Select **1/regions (peaks)** from the *Spreadsheet* drop-down menu
* Select **11. p-value(Sample ID vs. mock)** from the *Column* drop-down menu
* Select **significant with FDR of** from the *include p-values* drop-down menu with a value of **0.05**

![](/files/4rSLn8u3Rcem0BqidP4s)

Figure 2. Creating a criteria that includes regions significantly enriched in ChIP vs. mock

* Select **OK** to add the criteria to the criteria list (Figure 3)

![](/files/CXB9lzi38QzTqMWHa7r0)

Figure 3. New criteria are added to the criteria list

* Select **Save**
* Select **p-value filtered** from the list of criteria (Figure 4)

![](/files/q6DJVkYs7w7G12AysBNO)

Figure 4. Choosing criteria to save as lists

* Select **OK**

The new spreadsheet will open (Figure 5).

![](/files/CXtQaZGRurJsFQVhSAZc)

Figure 5. Spreadsheet with regions that are significantly enriched in the ChIP sample vs. control

Other *List Creator* operations like the *Venn Diagram,* *Union (Or),* and *Intersection (And)* of the lists could be used to create different lists of enriched peaks. For example, you could filter on the intersection between *Strand Separation FDR of 0.05* and *Peaks not in mock* or filter by *scaled fold change* or apply a minimum number of reads per million. The choice of what peaks you want to consider for downstream analysis depends on the goals and details of your experimental design.

## Additional Assistance

If you need additional assistance, please visit [our support page](http://www.partek.com/support) to submit a help ticket or find phone numbers for regional support.


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