# Create a marker list

To analyze differences in methylation between our experimental groups, we need to create a list of deferentially methylated loci.

* Select **Create Marker List** from the Analysis section of the *Illumina BeadArray Methylation* workflow
* Select LCLs vs. B cells (Figure 1)

![](https://1384254481-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FJVEESmJAPppJ3ijFq5aR%2Fuploads%2Fgit-blob-32bb7bc1f59ba62567d5f761068d8fa0571fcad5%2Fimage2017-12-26%2011_30_29.png?alt=media)

Figure 1. Creating a list of significantly differentially methylated loci

* Leave **Include size of the change** selected and set to *Change >* **2** *OR Change <* **-2**
* Leave **Include significance of the change** selected and set to **p-value with FDR** < **0.05**
* Select **Create**
* Select **Close** to exit the list manager

The new spreadsheet *LCLs vs. B cells* *(LCLs vs. B cells)* will open in the Analysis tab.

It is best practice to occasionally save the project you are working on. Let's take the opportunity to do this now.

* Select **File** from the main command toolbar
* Select **Save Project...**
* Specify a name for the project, we chose *Methylation Tutorial*, using the *Save File* dialog
* Select **Save** to save the project

Saving the project saves the identity and child-parent relationships of all spreadsheets displayed in the spreadsheet tree. This allows us to open all relevant spreadsheets for our analysis by selecting the project file.

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