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Process Xenium data

  • Obtain and add files to the project

  • Perform quality analysis / quality control and filtering

  • Normalize the data

Obtain and add files to the project

Follow along to add files to your Xenium project: Add files to the project.

Perform quality analysis / quality control and filtering

  • Filter the data including control probes using the Filter features task

  • Choose Feature metadata filter

  • Include the Gene Expression features

  • This results in a Filter features task (rectangle) and node (circle) results.

  • Right click the circle to Rename data node to "Filtered to only gene expression"

  • Click the circular "Filtered to only gene expression" results node and select the Single cell QA/QC task from the context sensitive menu on the right

  • When the task completes it will be opaque and no longer transparent with a progress bar

Double click the opaque rectangle task to open and Apply the observation filter to the "Filtered to only gene expression" results node. This results in a "Filtered cells node".

  • Select the "Filtered cells node" and choose the Normalization task from the Normalization and scaling drop-down in the task menu

  • Click the Use recommended button to proceed with these settings

  • Click Finish

This results in a Normalized counts node as shown below in the pipeline.

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Click Finish

Normalize the data

Additional Assistance

filter cells as described here.
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